Mitosis cell division stages

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Mitosis - cell division

Middle School Biology

Mitosis

Stages of mitosis

Right now, somewhere in your body, a cell is dividing. Its chromosomes are being pulled apart by protein fibers. Its nuclear envelope has dissolved. Two new nuclei are forming. Within minutes, one cell will have become two.

This process happens billions of times in your body every day. It was built from a single fertilized egg. It heals your wounds. It replaces your worn-out blood cells. It is one of the most precisely choreographed processes in all of biology.

What Is Mitosis?

Mitosis is a type of cell division that produces two genetically identical daughter cells, each with the same number of chromosomes as the parent cell.

If the parent cell is diploid (2n), containing two sets of chromosomes, each daughter cell is also diploid (2n). In humans, where body cells contain 46 chromosomes, mitosis produces daughter cells each with 46 chromosomes, genetically identical to the parent.

Mitosis is used for growth, repair, and cell replacement in multicellular organisms, and for asexual reproduction in some organisms.

Preparation: DNA Replication in Interphase

Before mitosis begins, the cell must copy all its DNA during the S phase of interphase.

After replication, each chromosome consists of two identical copies called chromatids, joined at a region called the centromere. These paired chromatids are called sister chromatids.

The cell now contains double the normal amount of DNA but still has the normal number of chromosomes, each consisting of two identical chromatids.

Prophase

Prophase is the first and longest stage of mitosis.

  • Chromosomes condense and become visible under a light microscope as they coil tightly.
  • Each chromosome is visible as two sister chromatids joined at the centromere.
  • The nuclear envelope begins to break down and disappears.
  • The spindle apparatus begins to form from centrioles (in animal cells).
  • Spindle fibers extend from centrioles at opposite poles of the cell.

Metaphase

Metaphase is the stage during which chromosomes are most clearly visible.

  • Spindle fibers attach to the centromere of each chromosome.
  • Chromosomes are pulled and pushed until they align along the equator (middle) of the cell.
  • This alignment is called the metaphase plate.
  • Each chromosome is attached to spindle fibers from both poles.

Metaphase is the stage at which chromosomes are most condensed and most clearly distinct, making it the best stage for counting chromosomes and studying their structure.

Anaphase

Anaphase is the stage during which chromosomes are separated.

  • The centromere of each chromosome splits.
  • Sister chromatids are pulled apart toward opposite poles of the cell by the shortening of spindle fibers.
  • Each separated chromatid is now considered an individual chromosome.
  • The cell elongates as spindle fibers push the poles apart.
  • At the end of anaphase, each pole has a complete set of chromosomes identical to the original.

Telophase

Telophase is the final stage of mitosis.

  • Chromosomes reach opposite poles.
  • Chromosomes begin to decondense (uncoil).
  • Nuclear envelopes reform around each set of chromosomes.
  • Spindle fibers break down.
  • Two nuclei are now present in the cell, each genetically identical to the original.

Cytokinesis

Cytokinesis is the division of the cytoplasm, which usually occurs alongside or immediately after telophase.

In animal cells: A contractile ring of protein filaments pinches the cell membrane inward at the equator, eventually dividing the cytoplasm into two. This produces a cleavage furrow that deepens until the cell is separated into two daughter cells.

In plant cells: A cell plate forms across the middle of the cell from the inside outward. The cell plate is composed of vesicles from the Golgi apparatus that contain cell wall material. The cell plate expands until it fuses with the existing cell wall, dividing the cell into two.

Summary of Mitotic Stages

Stage Key Events
ProphaseChromosomes condense, nuclear envelope breaks down, and spindle forms
MetaphaseChromosomes align at the cell equator, spindle fibers attach to centromeres
AnaphaseSister chromatids separate and move to opposite poles
TelophaseNuclear envelopes reform, chromosomes decondense
CytokinesisCytoplasm divides, two daughter cells formed

The Outcome of Mitosis

The two daughter cells produced by mitosis are:

  • Genetically identical to each other
  • Genetically identical to the parent cell
  • Diploid (same chromosome number as parent)
  • Each containing a complete, functional set of chromosomes

This genetic identity is essential for mitosis's roles in growth and repair. When a skin cell divides to heal a wound, the new cells must be genetically identical skin cells, not some other cell type.

Mitosis and Asexual Reproduction

Some organisms reproduce entirely through mitosis, producing offspring that are genetically identical to the parent. This is called asexual reproduction.

  • Bacteria reproduce by binary fission, a process equivalent to mitosis
  • Yeast reproduce by budding, where a small outgrowth forms through mitosis and separates
  • Hydra produce buds that grow by mitosis and separate
  • Many plants reproduce vegetatively through runners, bulbs, or tubers, all produced by mitosis

Asexual reproduction through mitosis is rapid and efficient. However, all offspring are genetically identical, providing no genetic variation for natural selection to act upon.

Control of Mitosis

Mitosis is carefully regulated. Cells normally divide only when signaled to do so by growth factors and only when conditions are appropriate.

Contact inhibition is the phenomenon by which cells stop dividing when they contact neighboring cells. This prevents overgrowth of tissues.

When the regulatory mechanisms controlling mitosis fail, cells may divide uncontrollably. This is the basis of cancer, where mutations in genes controlling the cell cycle lead to unregulated mitosis and tumor formation.