Cell division - mitosis and meiosis

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Cell cycle and division

Middle School Biology

Cell Division

Cell division process

Every second, your body produces approximately 3.8 million new cells. Skin cells that were shed are replaced. Red blood cells worn out after 120 days are renewed. Damaged tissues are repaired. A wound that was open yesterday begins to close today.

All of this depends on cell division, the process by which one cell produces two. It is one of the most fundamental processes in all of biology and the basis of growth, repair, reproduction, and the continuity of life.

Why Do Cells Divide?

Cell division serves three essential purposes in living organisms.

  • Growth: Multicellular organisms grow from a single fertilized cell into a complete organism containing trillions of cells through repeated rounds of cell division.
  • Repair and replacement: Worn-out, damaged, or dead cells are continuously replaced by new ones produced through cell division. The lining of the small intestine is completely replaced every few days. Red blood cells are replaced every four months.
  • Reproduction: Cell division is the basis of asexual reproduction in single-celled organisms. In multicellular organisms, it produces the specialized sex cells (gametes) needed for sexual reproduction.

The Cell Cycle

The cell cycle is the sequence of events a cell goes through from one division to the next.

It consists of two main phases.

Interphase

Interphase is the period between cell divisions. It is not a resting phase, despite sometimes being called one. During interphase, the cell is extremely active.

  • G1 phase (Gap 1): The cell grows in size, produces proteins and organelles, and prepares for DNA replication. The cell responds to signals from its environment that determine whether division will proceed.
  • S phase (Synthesis): DNA replication occurs. Every chromosome is copied exactly, producing two identical sister chromatids joined at the centromere. The amount of DNA in the cell doubles.
  • G2 phase (Gap 2): The cell continues to grow and produce proteins needed for division. The duplicated chromosomes begin to condense. The cell checks that DNA replication was completed accurately.

M Phase (Mitotic Phase)

The M phase includes nuclear division (mitosis or meiosis) and cytoplasmic division (cytokinesis).

DNA Replication

Before a cell can divide, it must copy all of its DNA so that each daughter cell receives a complete set of genetic instructions.

DNA replication is semi-conservative. Each new DNA molecule consists of one original strand and one newly synthesized complementary strand.

The process:

  1. The enzyme helicase unwinds the double helix and separates the two strands by breaking the hydrogen bonds between base pairs
  2. Each strand serves as a template
  3. The enzyme DNA polymerase reads each template strand and assembles a new complementary strand by adding free nucleotides according to base pairing rules (A with T, G with C)
  4. Two identical double-stranded DNA molecules are produced, each identical to the original

DNA replication is remarkably accurate. DNA polymerase also has a proofreading function, correcting most errors as they occur. Replication errors occur approximately once per billion base pairs copied.

Two Types of Cell Division

There are two fundamentally different types of cell division in eukaryotes.

Mitosis

Produces two daughter cells that are genetically identical to each other and to the parent cell. It is used for growth, repair, and asexual reproduction.

Meiosis

Produces four daughter cells each with half the chromosome number of the parent cell. The daughter cells are genetically different from each other and from the parent cell. It is used to produce gametes for sexual reproduction.

Control of the Cell Cycle

The cell cycle is tightly controlled at several checkpoints where the cell checks that conditions are right to proceed.

  • G1 checkpoint: Is the cell large enough? Is the environment favorable? Has any DNA damage been repaired?
  • G2 checkpoint: Has DNA been replicated correctly and completely? Is the cell large enough?
  • M checkpoint (spindle assembly checkpoint): Are all chromosomes properly attached to spindle fibers before they are separated?

Proteins called cyclins and cyclin-dependent kinases (CDKs) regulate progression through the cell cycle. Their levels rise and fall at specific points, triggering the transitions between phases.

If checkpoint controls fail, cells may divide uncontrollably. This is the fundamental basis of cancer.

Cancer: Cell Division Out of Control

Cancer results from mutations in genes that control the cell cycle.

  • Proto-oncogenes are normal genes that promote cell division. Mutations can convert them into oncogenes that continuously drive division.
  • Tumor suppressor genes normally apply the brakes to cell division and trigger apoptosis (programmed cell death) in damaged cells. Mutations can inactivate them, removing these controls.

When both types of control are lost, cells divide uncontrollably, forming tumors. If tumor cells acquire the ability to invade surrounding tissues and travel through blood or lymph to establish new tumors elsewhere (metastasis), the cancer becomes life-threatening.

Mutagens such as UV radiation, tobacco smoke, and certain viruses increase the rate of mutations in cell cycle control genes, which is why they are associated with increased cancer risk.

Apoptosis

Apoptosis is programmed cell death, a controlled process by which cells that are damaged, infected, or no longer needed are systematically dismantled and removed.

Apoptosis is essential for normal development and tissue homeostasis. During development it sculpts tissues. The spaces between fingers and toes form because cells in between die by apoptosis.

Apoptosis is also the mechanism by which the immune system kills infected cells and by which cells with irreparable DNA damage are eliminated before they can become cancerous.